Elucidating isoniazid resistance using molecular
The results show that the hydrogen bonding and van der Waals forces play major roles in stabilizing the 1:1 INH-SOD complex.
After addition of INH during the range of the experiment, the conformation and microenvironment of Cu/Zn-SOD are changed, but the activity of Cu/Zn-SOD is not changed. Isoniazid (INH) is the foremost first-line antibiotic used to treat TB, which continues to be the cornerstone of all antituberculosis regimens and remains the only agent recommended for tuberculosis chemoprophylaxis for children .
ATDH can be fatal if it is not recognised at an early stage, after which therapy should be interrupted timely.
Moreover, ATDH has a negative impact on therapy adherence, decreases success rates of treatment and eventually increases treatment failure, relapse or the emergence of drug resistance.
shows the fluorescence spectra of Cu/Zn-SOD upon the addition of INH.
Synchronous fluorescence measurement provides information about the molecular microenvironment in the vicinity of the fluorophore functional groups [, the fluorescence intensities of tryptophan residue and tyrosine residue decreased with the increase of the concentration of INH.
The maximum emission wavelength of tyrosine residues does not undergo a significant shift in the investigated concentration range.
The occurrence of hepatotoxicity related to INH has been well-defined .
To better understand the protective mechanism of SOD, the interaction between SOD and INH should be investigated.